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1301. Which of the following is subterranean water?
A. Atmospheric water
B. Surface water
C. Groundwater
D. Rainwater
1302. _____ is produced by Streptomyces rimosus.
A. Chlortetracycline
B. Oxytetracycline
C. Tetracycline
D. Doxycycline
1303. Solutions of sodium hypochlorite of a 1% concentration are used for ______.
A. Sanitizing dairy equipment
B. Personal hygiene and a household disinfectant
C. Household bleaches
D. Disinfecting open wounds
1304. Which was the first disease for which a chemotherapeutic agent was used?
A. Syphilis
B. Malaria
C. Small pox
D. AIDS
1305. The antibiotic penicillin was discovered by which of the following researchers?
A. Emmerich and Low
B. Gratia and Dath
C. Alexander Flemming
D. Robert Koch
1306. Cell-wall biosynthesis is inhibited by antibiotics by inhibiting the biosynthesis of which of the following?
A. Lipopolysaccharide
B. Cellulose
C. Peptidoglycan
D. Proteins
1307. Benzylpenicillin is the chemical name for which of the following penicillin?
A. Penicillin G
B. Penicillin V
C. Penicillin F
D. Phenethicilin
1308. Streptomyces orientalis produces which of the following antibiotics?
A. Cephalosporins
B. Cycloserine
C. Bacitracin
D. Vancomycin
1309. Polymyxin inhibits the growth of the microbes by carrying out which of the following actions?
A. Inhibition of cell-wall synthesis
B. Damage to cytoplasmic membrane
C. Inhibition of nucleic acid and protein synthesis
D. Inhibition of specific enzyme systems
1310. _________ are the diverse collection of organisms that live in large bodies of water and are unable to swim against a current.
A. Plankton
B. Phytoplankton
C. Zooplankton
D. Mycoplankton
1311. Which of the following techniques can be used to separate different-size fractions of the aquatic microbial community?
A. Submerged-slide technique
B. Fluorescent antibody technique
C. Membrane-filter technique
D. By measuring biochemical activity
1312. In the high-temperature short-time (HTST) method of pasteurization, milk is exposed to a temperature of _________.
A. 32-degree C
B. 45-degree C
C. 63-degree C
D. 72-degree C
1313. Which of the following types of association is present among Staphylococcus aureus and Aspergillus terreous?
A. Antagonism
B. Mutualism
C. Parasitism
D. Commensalism
1314. Which of the following inhibits protein synthesis by combining with the 50S subunit ribosome?
A. Streptomycin
B. Tetracycline
C. Chloramphenicol
D. Penicillin
1315. Which of the following is the most accurate method for microbial assay of antibiotics?
A. Physical assay
B. Chemical assay
C. Biological assay
D. Chemical and biological assay
1316. Pigs respond dramatically to the addition of which of the following antibiotics to their diet?
A. Bacitracin
B. Polymyxin
C. Penicillin
D. Oxytetracycline
1317. Which of the following antibiotic have a sparing effect on the B12 in the diet?
A. Streptomycin
B. Tetracycline
C. Polymyxin
D. Chloramphenicol
1318. Lytic enzymes which destroy are secreted by which of the following microorganism?
A. Fungi
B. Algae
C. Staphylococcus
D. Myxobacteria
1319. Streptomycin is produced by which of the following organisms?
A. Streptomyces noursei
B. Streptomyces nodosus
C. Streptomyces fradiae
D. Streptomyces griseus
1320. ______ is a symbiotic nitrogen fixing bacteria.
A. Rhizobium
B. Clostridium
C. Azotobacter.
D. Staphylococcus
1321. In carbon cycle flow of energy is ________.
A. Bidirectional
B. Linear
C. Cyclic
D. Irreversible
1322. Acycloguanosine is a nucleoside analog which is active against ___________________.
A. Influenza A virus
B. HIV virus
C. Herpes virus
D. Influenza B virus
1323. Which of the following has its antiviral action attributed to interference of protein synthesis?
A. Amantadine
B. Interferons
C. Acycloguanosine
D. Bacteriocin
1324. Which of the following is effective in the control of tuberculosis in humans?
A. Nitrofurans
B. Nalidixic Acid
C. Sibromycin
D. Isoniazid
1325. Anthramycin is which of the following type of antibiotic?
A. Antiviral
B. Antitumor
C. Antifungal
D. Antibacterial
1326. Pasteurization of milk takes place at _______.
A. 55oC
B. 37oC
C. 63oC
D. 45oC
1327. What will be plate count of good quality water?
A. 10 per ml
B. 50 per ml
C. 100 per ml
D. 1000 per ml
1328. Which of the following is not a characteristic of a chemotherapeutic agent?
A. Low toxicity for host cells
B. High toxicity for the parasites
C. Less penetrating power
D. Does not affect the host’s natural defense mechanism
1329. ___ inhibitory toxic product is produced by fungi.
A. Cyanide
B. Fatty acids
C. Methane
D. Sulphides
1330. Which of the following comes under the category of positive association?
A. Neutralism
B. Parasitism
C. Commensalism
D. Ammensalism
1331. Which of the following organisms are known to grow on the surfaces of freshly exposed rocks?
A. Green algae
B. Diatoms
C. Cyanobacteria
D. Yeast
1332. The transformation of nitrates to gaseous nitrogen is known as ___________.
A. Ammonification
B. Nitrification
C. Reduction of nitrate to ammonia
D. Denitrification
1333. Which of the following conditions decreases the level of denitrification?
A. Abundance of organic matter
B. Acidic pH
C. Elevated temperatures
D. Availability of oxygen
1334. Green plants and _______ are the most important agents of carbon dioxide fixation.
A. Bacteria
B. Fungi
C. Algae
D. Protozoa
1335. Fresh air contains approximately ______ percent carbon dioxide by volume.
A. 0.001
B. 2
C. 5
D. 0.03
1336. Cellulose is degraded to cellobiose by ________.
A. Cellulase
B. Beta-glucosidase
C. Hexokinase
D. Cellulose dehydrogenase
1337. The surface water is susceptible to contamination with microorganisms from ___________.
A. The air
B. The surface runoff
C. Precipitation
D. Atmospheric water and the surface runoff
1338. Thermus aquaticus, a common bacterial inhabitant generally prevails in temperatures of ________.
A. 00oC to 05oC
B. 30 oC to 40 oC
C. 70 oC to 72 oC
D. 90 oC to 95oC
1339. Primary producers are found growing in which of the following layers of water?
A. Upper layer
B. Middle layer
C. Intermediate layer
D. Bottom layer
1340. The microorganisms from lakes and rivers can grow at a salt concentration of _________.
A. 2.5 to 4 percent
B. 5 percent
C. Below 1 percent
D. Above 1 percent
1341. Which among the following is a gas producer?
A. Streptococcus lactis
B. Lactobacillus fermentum
C. Micrococcus luteus
D. Clostridium butyricum
1342. Which of the following compounds are required for growth of saprophytic bacteria and fungi?
A. Organic compounds
B. Nitrates
C. Phosphates
D. Mercury
1343. The moisture contained in clouds and precipitated as snow, hail, rain etc. constitutes ___________.
A. Atmospheric water
B. Surface water
C. Groundwater
D. Subterranean water
1344. Which of the following genus of bacteria is NOT found in fresh waters?
A. Pseudomonas
B. Flavobacterium
C. Aeromonas
D. Vibrio
1345. _____ form of water is the most contaminated.
A. Underground water
B. Rainwater
C. Surface water
D. Water stored in ice caps
1346. Alum is used for which of the following purification process?
A. Sedimentation
B. Filtration
C. Disinfection
D. Ozonation
1347. The coliform group of bacteria includes all the __________________ bacilli.
A. Aerobic
B. Anaerobic
C. Aerobic, non-sporulating
D. Aerobic, Gram-negative, non-sporulating
1348. Which of the following organism does not belong to coliform group of bacteria?
A. Salmonella
B. Klebsiella
C. Enterobacter
D. Proteus
1349. Among the following which microorganism acts as a representative of fecal streptococci group?
A. Streptococcus faecalis
B. Streptococcus bovis
C. Streptococcus equinus
D. Streptococcus faecium
1350. Which of the following sewerage systems carry domestic and industrial wastewater?
A. Sanitary sewers
B. Storm sewers
C. Combined sewers
D. Storm and combined sewers
1351. In which of the following treatment involve oxidation of organic constituents of the wastewater?
A. Primary treatment
B. Secondary treatment
C. Advanced treatment
D. Final treatment
1352. Acetic acid and lactic acid are used for _________.
A. Curing meats
B. Preservation of color
C. Preservation of pickles
D. Inhibiting mold growth
1353. Microbial inhabitants of the bottom region of a body of water are referred to as _________.
A. Phytoplankton
B. Benthic organisms
C. Zooplankton
D. Mycoplankton
1354. The upper region of the trickling filter is favorable for the growth of _____________.
A. Fungi
B. Protozoa
C. Algae
D. Bacteria
1355. Which of the following gases are produced in large amounts during sludge digestion?
A. Methane
B. Carbon-dioxide
C. Hydrogen
D. Nitrogen
1356. Which of the following organism occurs on the skin of fresh poultry?
A. Pseudomonads
B. Staphylococci
C. Micrococci
D. Enterococci
1357. Which among following group of microorganisms are found in the ducts of cow’s mammary glands?
A. Coliforms
B. Lactobacilli
C. Micrococci
D. Mycobacteria
1358. The coloration of the milk is caused by which of the following organism?
A. Pseudomonas sp.
B. Bacillus sp.
C. Penicillium sp.
D. Streptococcus sp.
1359. Which of the following type of spoilage occurs in fresh meat?
A. Souring
B. Greening
C. Putrefaction
D. Moldy
1360. Spoilage of bread is caused by which of the following microorganism?
A. Saccharomyces
B. Rhizopus
C. Leuconostoc
D. Pseudomonas
1361. Temperatures used for canning foods ranges from:
A. 0-20-degree C
B. 20-60-degree C
C. 60-100-degree C
D. 100-121-degree C
1362. ____ microorganism survive at -9 to -17-degree C?
A. Salmonella
B. Staphylococcus
C. Bacillus
D. Clostridium
1363. Which of the following microorganism should be eliminated in canned foods?
A. Mycobacterium tuberculosis
B. Coxiella burnetii
C. Clostridium botulinum
D. Lactobacillus
1364. Phosphatase enzyme present in milk is destroyed in which of the following processes?
A. Sterilization
B. Canning
C. Dehydration
D. Pasteurization
1365. Which of the following microbe is used in the production of blue cheese?
A. Streptococcus thermophilus
B. Lactobacillus bulgaricus
C. Penicillium roqueforti
D. Rhizopus stolonifer
1366. Bacterial cell grown on hydrocarbon wastes from the petroleum industry are a source of ________.
A. Carbohydrates
B. Proteins
C. Vitamins
D. Fats
1367. _____ microorganism have a high vitamin content.
A. Bacteria
B. Yeast
C. Algae
D. Protozoa
1368. Shredded cabbage is the starting product for which of the following fermented food?
A. Sauerkraut
B. Pickles
C. Green olives
D. Sausage
1369. _____ product utilizes whey as its raw material.
A. Lactic acid
B. Acetic acid
C. Glutamic acid
D. Lysine
1370. Which of the following is NOT a desired characteristic of the organism to be used for industrial application?
A. Should be readily available
B. Should grow rapidly
C. Should produce less amount of product
D. Should be nonpathogenic
1371. Solvents and enzymes are found in which of the following categories of microbial products?
A. Pharmaceutical chemicals
B. Commercially valuable chemicals
C. Food supplements
D. Alcoholic beverages
1372. Which of the following enzymes cut the DNA molecule at particular nucleotide sequence?
A. Restriction endonuclease
B. Exonuclease
C. Ligase
D. Polymerase
1373. ______ is used for the treatment of blood clots?
A. Insulin
B. Interferon
C. Urokinase
D. Somatostatin
1374. ______ is used to inhibit mold growth in bread.
A. Benzoic acid
B. Nitrates
C. Sorbic acid
D. Lactic acid
1375. VP3 protein is isolated from:
A. TMV
B. HIV
C. HRV
D. FMDV
1376. Which of the following carbohydrates are mainly present in whey?
A. Glucose
B. Lactose
C. Fructose
D. Sucrose
1377. What temperature is necessary for the production of vinegar?
A. 43-degree C
B. 60-degree C
C. 10-13-degree C
D. 15-34-degree C
1378. Diaminopimelic acid (DAP) is produced by which of the following microorganism?
A. E. coli
B. Enterobacter aerogenes
C. Bacillus subtilis
D. Streptococcus equisimilis
1379. Insulin was isolated from which of the following organs of animals?
A. Small intestine
B. Tongue
C. Pancreas
D. Stomach
1380. Which among the following are NOT used as raw materials for alcohol production?
A. Corn
B. Molasses
C. Whey
D. Grapes
1381. What is the pH required for production of baker’s yeast?
A. 1 to 2
B. 4 to 5
C. 7 to 8
D. 10 to 12
1382. The leavening or rising of dough is due to which of the following gases?
A. Oxygen
B. Carbon dioxide
C. Hydrogen
D. Sulphur dioxide
1383. Which of the following instrument is used for recovery of yeast cells?
A. Fermenter
B. Centrifuge
C. Filter press
D. Mash storage
1384. Which of the following product is used as a food condiment?
A. Baker’s yeast
B. Ethanol
C. Wine
D. Soy sauce
1385. Gluconic acid is produced by which of the following molds?
A. Aspergillus niger
B. Rhizopus nigricans
C. Aspergillus terreus
D. Rhizopus oryzae
1386. Fermentation of rice is carried out by which of the following microorganisms?
A. Bacteria
B. Yeasts
C. Molds
D. Protozoa
1387. Which of the following techniques is used to improve penicillin production?
A. Fed-batch fermentation
B. Submerged-culture technique
C. Continuous fermentation
D. Batch fermentation
1388. Which of the following product acts as a substrate for the production of citric acid?
A. Molasses
B. Corn-steep liquor
C. Barn
D. Soyabeen meal
1389. Which of the following enzymes is used to hydrolyze starch to dextrin?
A. Invertase
B. Pectinase
C. Amylase
D. Protease
1390. Which of the following enzyme is used in the process of bating?
A. Invertase
B. Pectinase
C. Amylase
D. Protease
1391. Invertase causes hydrolysis of ___________.
A. Starch
B. Sucrose
C. Proteins
D. Pectins
1392. Rhizopus nigricans produces which of the following products?
A. Citric acid
B. Fumaric acid
C. Gibberellic acid
D. Lactic acid
1393. Which is the functional unit of inheritance?
A. DNA
B. Chromosome
C. Gene
D. RNA
1394. Populations of cells descending from a single cell are known as ____________.
A. Mutants
B. Clones
C. Wild-type cells
D. Parent cell
1395. The replacement of a purine by a pyrimidine or vice versa is known as ____________.
A. Transversion
B. Transition
C. Base-pair substitution
D. Missense mutation
1396. Sickle cell anemia is which type of mutation?
A. Nonsense mutation
B. Missense mutation
C. Deletion mutation
D. Insertion mutation
1397. Which of the following mutations result in frameshift mutation?
A. Missense mutation
B. Transition
C. Transversion
D. Insertion
1398. Which of the following mutagen causes a break in the phosphodiester backbone of the nucleic acid?
A. UV light
B. X-rays
C. Nitrous acid
D. Base analogs
1399. Photoreactivation can cause repair of DNA damaged by which of the following mutagens?
A. UV rays
B. X-rays
C. Nitrous acid
D. Base analogs
1400. Which of the following enzyme is used to join the DNA fragments together?
A. Polymerases
B. Exonuclease
C. Endonuclease
D. Ligase
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