Histopathology mcq with answer
Specimen Processing MCQs
- What is the first step in specimen processing?
- A. Embedding
- B. Fixation ✅
- C. Sectioning
- D. Staining
- Which fixative is most commonly used in histology?
- A. Ethanol
- B. Formalin ✅
- C. Acetone
- D. Paraffin
- What is the purpose of dehydration in specimen processing?
- A. To remove fat
- B. To prepare tissue for embedding ✅
- C. To dissolve proteins
- D. To stain the tissue
- Which chemical is commonly used for tissue dehydration?
- A. Xylene
- B. Ethanol ✅
- C. Formalin
- D. Acetone
- What is the role of paraffin in specimen processing?
- A. Fixing the tissue
- B. Embedding the tissue ✅
- C. Staining the tissue
- D. Clearing the tissue
- Clearing is done to:
- A. Remove water from the tissue
- B. Make the tissue transparent ✅
- C. Harden the tissue
- D. Embed the tissue
- What temperature is generally used for paraffin embedding?
- A. 37°C
- B. 45°C
- C. 58°C ✅
- D. 72°C
- What is the purpose of sectioning in specimen processing?
- A. To cut tissue into thin slices ✅
- B. To fix the tissue
- C. To stain the tissue
- D. To remove excess tissue
- Which instrument is used for sectioning tissue?
- A. Microtome ✅
- B. Cryostat
- C. Ultracentrifuge
- D. Spectrophotometer
- Why is mounting performed in specimen processing?
- A. To fix the tissue on the slide
- B. To protect the tissue for microscopic analysis ✅
- C. To dehydrate the tissue
- D. To make the tissue transparent
- Which mounting medium is commonly used in histology?
- A. DPX ✅
- B. Xylene
- C. Glycerol
- D. Paraffin
- What is the purpose of staining in histological processing?
- A. To fix the tissue
- B. To enhance contrast for microscopic analysis ✅
- C. To remove water
- D. To embed the tissue
- Which chemical is used for tissue clearing?
- A. Formalin
- B. Xylene ✅
- C. Acetone
- D. DPX
- What is a critical factor to ensure optimal tissue processing?
- A. Proper fixation ✅
- B. Avoiding dehydration
- C. Rapid clearing
- D. Using distilled water
- Why is embedding important in histological processing?
- A. To preserve tissue morphology ✅
- B. To fix tissue
- C. To enhance staining
- D. To section tissue
