Histopathology Techniques and Tissue Processing MCQs
- What is the primary purpose of tissue fixation in histology?
- A. To enhance staining
- B. To preserve tissue structure and prevent decomposition ✅
- C. To increase tissue hydration
- D. To remove unwanted cells
- Which fixative is most commonly used in routine histopathological work?
- A. Acetone
- B. Formalin ✅
- C. Alcohol
- D. Glutaraldehyde
- What is the purpose of embedding in tissue processing?
- A. To freeze the tissue for later use
- B. To support and harden the tissue for sectioning ✅
- C. To stain the tissue
- D. To preserve cellular components
- In the process of dehydration, which of the following solutions is commonly used?
- A. Xylene
- B. Ethanol ✅
- C. Formalin
- D. Acetone
- Which tissue embedding medium is most commonly used for routine histopathology?
- A. Paraffin wax ✅
- B. Resin
- C. Gelatin
- D. Cryo-embedding media
- What is the function of the microtome in tissue processing?
- A. To fix the tissue
- B. To cut tissue into thin sections ✅
- C. To stain the tissue
- D. To dehydrate the tissue
- What is the typical thickness of a tissue section cut using a microtome?
- A. 10-20 micrometers ✅
- B. 30-50 micrometers
- C. 5-10 micrometers
- D. 100 micrometers
- Which of the following is the primary purpose of clearing in tissue processing?
- A. To remove excess fixative
- B. To replace alcohol with a substance compatible with paraffin ✅
- C. To preserve tissue structure
- D. To stain tissue
- Which method is commonly used for rapid tissue processing?
- A. Microwave processing ✅
- B. Traditional overnight processing
- C. Freeze-drying
- D. Cryopreservation
- What is the role of the paraffin wax in tissue processing?
- A. To harden the tissue for easier sectioning ✅
- B. To preserve cellular structures
- C. To improve staining quality
- D. To remove excess fixative
- What is the principle behind the use of frozen sections in histopathology?
- A. To quickly preserve tissue in its natural state
- B. To quickly obtain thin sections for diagnosis without processing in paraffin ✅
- C. To enhance staining
- D. To remove formalin fixation
- Which of the following is used to remove paraffin wax from tissue sections?
- A. Ethanol
- B. Xylene ✅
- C. Water
- D. Acetone
- What is the significance of using different concentrations of alcohol during dehydration?
- A. To adjust the viscosity of the tissue
- B. To gradually remove water without damaging tissue structure ✅
- C. To enhance tissue staining
- D. To preserve cellular details
- In tissue processing, which of the following chemicals is used for the fixation of tissue?
- A. Ethanol
- B. Formalin ✅
- C. Paraffin
- D. Acetone
- Which of the following is an important step after embedding tissue in paraffin?
- A. Dehydration
- B. Clearing
- C. Sectioning ✅
- D. Fixation
