Histopathological Staining Techniques MCQs
- What is the purpose of histological staining?
- A. To enhance tissue preservation
- B. To differentiate between different tissue components ✅
- C. To remove excess fixative
- D. To harden the tissue
- Which stain is commonly used for routine tissue examination?
- A. Hematoxylin and eosin (H&E) ✅
- B. Wright’s stain
- C. Giemsa stain
- D. Gram stain
- In the H&E staining technique, what is the function of eosin?
- A. To stain DNA blue
- B. To stain cytoplasm and extracellular matrix pink ✅
- C. To stain proteins yellow
- D. To stain mitochondria purple
- What does hematoxylin stain in tissue samples?
- A. Collagen fibers
- B. Nuclei ✅
- C. Cytoplasm
- D. Mitochondria
- Which of the following is a key difference between acidic and basic stains?
- A. Acidic stains bind to positively charged cellular components, while basic stains bind to negatively charged components ✅
- B. Acidic stains bind to proteins, while basic stains bind to lipids
- C. Basic stains are used for living cells, while acidic stains are for dead cells
- D. Acidic stains are used for tissue fixation, while basic stains are used for sectioning
- Which of the following is a commonly used basic dye in histology?
- A. Eosin
- B. Methylene blue ✅
- C. Safranin
- D. Congo red
- What is the main purpose of using special stains in histology?
- A. To increase magnification
- B. To differentiate specific tissue components or structures that are not visible with H&E staining ✅
- C. To preserve tissue integrity
- D. To reduce the need for tissue sectioning
- Which of the following stains is used for identifying mycobacteria, such as in tuberculosis?
- A. Gram stain
- B. Ziehl-Neelsen stain ✅
- C. Giemsa stain
- D. Wright’s stain
- In which type of tissue does Periodic Acid-Schiff (PAS) staining reveal glycogen and mucopolysaccharides?
- A. Muscle tissue
- B. Connective tissue
- C. Epithelial tissue ✅
- D. Nervous tissue
- Which stain is commonly used for identifying connective tissue fibers?
- A. Van Gieson’s stain ✅
- B. Giemsa stain
- C. Oil red O stain
- D. Sudan black B stain
- What is the main application of immunohistochemistry (IHC) in histopathology?
- A. To examine cell structure
- B. To detect specific antigens or proteins in tissue samples ✅
- C. To preserve tissue morphology
- D. To stain cell membranes
- Which type of dye is used in the Masson’s trichrome stain for detecting collagen?
- A. Acid fuchsin
- B. Aniline blue ✅
- C. Safranin
- D. Eosin
- What is the function of the Gram stain in histopathology?
- A. To identify bacterial cell walls as either Gram-positive or Gram-negative ✅
- B. To identify DNA in the nucleus
- C. To highlight the cytoplasm
- D. To stain mucin
- What is the role of eosin in the Masson’s trichrome stain?
- A. To stain collagen fibers red
- B. To stain the cytoplasm of cells pink ✅
- C. To stain mucin green
- D. To stain the muscle fibers blue
- In which situation is Oil Red O used in histology?
- A. To detect protein deposits
- B. To detect lipids in tissue sections ✅
- C. To stain collagen fibers
- D. To identify cell nuclei
