Cryosectioning MCQs
- What is the main purpose of cryosectioning?
- A. To fix tissues permanently
- B. To rapidly freeze and cut tissues for microscopic examination ✅
- C. To prepare tissues for paraffin embedding
- D. To dehydrate tissues
- What type of instrument is used in cryosectioning?
- A. Microtome
- B. Cryostat ✅
- C. Centrifuge
- D. Thermocycler
- What is the ideal temperature range for cryosectioning?
- A. -5°C to -10°C
- B. -10°C to -20°C ✅
- C. -30°C to -40°C
- D. -40°C to -50°C
- What is the primary advantage of cryosectioning over paraffin embedding?
- A. Better long-term preservation of tissues
- B. Faster processing time ✅
- C. Enhanced nuclear detail
- D. Reduced reagent usage
- What is the purpose of OCT compound in cryosectioning?
- A. To fix tissues
- B. To act as a freezing medium ✅
- C. To stain tissues
- D. To section tissues
- What is the most common type of tissue used in cryosectioning?
- A. Fixed tissues
- B. Fresh or frozen tissues ✅
- C. Dehydrated tissues
- D. Paraffin-embedded tissues
- Which staining technique is often performed on cryosections?
- A. Hematoxylin and Eosin (H&E) ✅
- B. Gram staining
- C. Acid-fast staining
- D. Periodic acid–Schiff (PAS)
- What is a major drawback of cryosectioning?
- A. Slow processing time
- B. Poor tissue preservation ✅
- C. High costs
- D. Complex equipment
- Why is rapid freezing critical in cryosectioning?
- A. To ensure tissue dehydration
- B. To prevent ice crystal formation ✅
- C. To improve nuclear staining
- D. To enhance tissue rigidity
- . What substance is often used for rapid freezing in cryosectioning?
- A. Liquid nitrogen ✅
- B. Ethanol
- C. Xylene
- D. Acetone
- . What thickness of tissue sections is commonly used in cryosectioning?
- A. 1-2 μm
- B. 5-10 μm ✅
- C. 20-30 μm
- D. 50-100 μm
- . How can tissue folding during cryosectioning be minimized?
- A. By warming the tissue block
- B. By using a fresh blade ✅
- C. By increasing the section thickness
- D. By reducing the freezing temperature
- Why are cryosections commonly used for enzyme histochemistry?
- A. Enzymes are preserved better in frozen sections ✅
- B. Paraffin embedding damages enzymes
- C. Frozen tissues provide better nuclear detail
- D. It requires less processing time
- What is the most common artifact seen in cryosections?
- A. Tissue shrinkage
- B. Ice crystal formation ✅
- C. Overstaining
- D. Poor contrast
- What can be done to improve adhesion of cryosections to slides?
- A. Use of charged slides ✅
- B. Use of thicker sections
- C. Reducing freezing time
- D. Avoiding OCT compound
