Histopathology and Microscopy Techniques MCQs
- What is the primary function of a light microscope in histopathology?
- A. To enhance tissue fixation
- B. To magnify tissue sections for examination ✅
- C. To stain tissue
- D. To preserve tissue structure
- Which microscope is used to examine fluorescently labeled tissue sections?
- A. Light microscope
- B. Electron microscope
- C. Confocal microscope ✅
- D. Phase-contrast microscope
- What is the advantage of using an electron microscope in histopathology?
- A. High magnification and resolution for observing cellular ultrastructure ✅
- B. It is easier to operate than light microscopes
- C. It allows for live cell imaging
- D. It uses visible light to examine tissue sections
- What is the purpose of phase contrast microscopy in histopathology?
- A. To observe cells in their natural state without staining ✅
- B. To examine tissue samples after fixation
- C. To detect fluorescence in tissue
- D. To provide high-resolution imaging of tissue ultrastructure
- In histopathology, what is the typical use of a cryostat?
- A. To quickly freeze tissue for sectioning ✅
- B. To enhance the tissue fixation process
- C. To prepare tissue for staining
- D. To observe tissue at high magnification
- What is the purpose of using a microtome in microscopy?
- A. To section tissue into thin slices for examination under a microscope ✅
- B. To magnify tissue sections
- C. To increase the resolution of the microscope
- D. To preserve tissue structure during processing
- Which type of microscope allows for the examination of tissue in three dimensions?
- A. Light microscope
- B. Scanning electron microscope (SEM) ✅
- C. Transmission electron microscope (TEM)
- D. Confocal microscope
- In electron microscopy, which component of the cell is best visualized?
- A. Cell wall
- B. Nucleolus
- C. Ultrastructure of organelles (e.g., mitochondria, endoplasmic reticulum) ✅
- D. Chromatin
- What is the main advantage of using a confocal microscope over a regular light microscope?
- A. Higher magnification
- B. Ability to produce three-dimensional images of tissue sections ✅
- C. Easier to use
- D. Ability to view live cells in real-time
- Which of the following techniques is commonly used for examining tissue sections at high magnification?
- A. Scanning electron microscopy (SEM)
- B. Transmission electron microscopy (TEM) ✅
- C. Confocal microscopy
- D. Phase contrast microscopy
- What does immunofluorescence microscopy allow researchers to detect?
- A. The presence of collagen fibers
- B. Specific proteins or antigens in tissue samples ✅
- C. The overall tissue structure
- D. The cell membrane composition
- Which part of the cell is commonly stained using the PAS (Periodic Acid-Schiff) technique?
- A. Nucleus
- B. Cytoplasm
- C. Glycogen and mucopolysaccharides ✅
- D. Cell membrane
- In histopathology, what is the primary use of oil immersion in microscopy?
- A. To enhance the color of tissue samples
- B. To increase the resolution by preventing light refraction ✅
- C. To preserve tissue structure
- D. To reduce the time required for tissue examination
- What is the purpose of staining tissue sections before observing them under a microscope?
- A. To remove excess fixative
- B. To enhance the contrast and differentiate tissue components ✅
- C. To improve the resolution of the microscope
- D. To increase tissue sectioning speed
- Which microscopy technique is best suited for visualizing tissue at a molecular level?
- A. Fluorescence microscopy ✅
- B. Phase contrast microscopy
- C. Light microscopy
- D. Confocal microscopy
