Fluorescence Microscopy MCQs
- What is the primary principle behind fluorescence microscopy?
- A. Light absorption and reflection
- B. Emission of light by fluorescent molecules ✅
- C. Scattering of light
- D. Polarization of light
- Which of the following is a commonly used fluorescent dye?
- A. Hematoxylin
- B. Eosin
- C. FITC (Fluorescein isothiocyanate) ✅
- D. Xylene
- What type of light source is typically used in fluorescence microscopy?
- A. Tungsten lamp
- B. LED light
- C. UV light ✅
- D. Halogen light
- What is the role of a dichroic mirror in fluorescence microscopy?
- A. To focus light onto the sample
- B. To reflect and transmit specific wavelengths of light ✅
- C. To magnify the image
- D. To eliminate background fluorescence
- Which of the following is a limitation of fluorescence microscopy?
- A. High cost
- B. Photo-bleaching of fluorescent dyes ✅
- C. Inability to visualize live cells
- D. Low magnification
- Which component enhances the specificity of fluorescence microscopy?
- A. Objective lens
- B. Excitation filter ✅
- C. Cover slip
- D. Staining chamber
- What is the main application of fluorescence microscopy in medical laboratories?
- A. Identifying histological structures
- B. Detecting specific proteins or molecules ✅
- C. Observing organ morphology
- D. Analyzing lipid metabolism
- What is the term for the emission of light by a substance after absorbing light?
- A. Reflection
- B. Fluorescence ✅
- C. Refraction
- D. Absorption
- Which part of the fluorescence microscope separates emitted light from the excitation light?
- A. Objective lens
- B. Barrier filter ✅
- C. Condenser
- D. Light source
- What is the typical lifespan of a fluorescent dye during microscopy?
- A. Unlimited
- B. Several hours
- C. Few minutes before photobleaching ✅
- D. Several days
- Which of the following is NOT a type of fluorescence microscope?
- A. Confocal fluorescence microscope
- B. Wide-field fluorescence microscope
- C. Atomic force microscope ✅
- D. Total internal reflection fluorescence microscope
- How is autofluorescence in biological samples minimized?
- A. Using brighter light sources
- B. Using shorter exposure times ✅
- C. Increasing the amount of stain
- D. Removing excitation filters
- What is a common application of fluorescence microscopy in histopathology?
- A. Staining connective tissues
- B. Identifying antibody-antigen interactions ✅
- C. Observing cellular morphology
- D. Analyzing bone tissue
- Which fluorescent dye is commonly used for nucleic acid staining?
- A. DAPI ✅
- B. Eosin
- C. Hematoxylin
- D. Rhodamine
- What is the advantage of confocal fluorescence microscopy over conventional fluorescence microscopy?
- A. Faster image acquisition
- B. Enhanced resolution and depth selectivity ✅
- C. Lower cost
- D. Simpler operation
