Histopathological Techniques MCQs
- What is the first step in preparing a tissue sample for histopathology?
- A. Staining the tissue
- B. Embedding the tissue in paraffin
- C. Fixing the tissue to preserve its structure ✅
- D. Cutting the tissue into thin slices
- Which chemical is most commonly used for tissue fixation in histopathology?
- A. Acetone
- B. Formaldehyde ✅
- C. Alcohol
- D. Xylene
- Why is embedding tissue in paraffin important for histopathology?
- A. It preserves the tissue color
- B. It allows for thin sectioning of the tissue ✅
- C. It enhances tissue staining
- D. It removes excess water from the tissue
- In histopathology, what is the purpose of decalcifying bone tissue before processing?
- A. To preserve bone structure
- B. To soften the bone for easier cutting ✅
- C. To enhance tissue staining
- D. To remove blood from the tissue
- What is the purpose of using xylene in histopathological techniques?
- A. To remove lipids from tissue samples
- B. To dehydrate the tissue
- C. To clear the tissue after dehydration ✅
- D. To fix tissue to glass slides
- Which of the following is the correct sequence of steps for preparing a tissue section for microscopy?
- A. Fixation → Embedding → Sectioning → Staining ✅
- B. Sectioning → Staining → Fixation → Embedding
- C. Staining → Sectioning → Embedding → Fixation
- D. Embedding → Sectioning → Fixation → Staining
- What is the most common method of sectioning tissue for microscopic examination?
- A. Cryosectioning
- B. Microtomy ✅
- C. Laser microdissection
- D. Ultrathin sectioning
- Why is the use of a microtome important in histopathology?
- A. To embed tissue in paraffin
- B. To cut tissue into thin slices for examination ✅
- C. To fix tissue to microscope slides
- D. To dehydrate tissue samples
- What is the purpose of staining tissue sections in histopathology?
- A. To preserve tissue structure
- B. To enhance the contrast of tissue components for better visualization ✅
- C. To remove excess water from tissue
- D. To reduce the time required for tissue sectioning
- Which of the following is the most commonly used stain in histopathology?
- A. Hematoxylin and eosin (H&E) ✅
- B. Periodic acid-Schiff (PAS)
- C. Wright’s stain
- D. Giemsa stain
- Which of the following is a common feature of tissues stained with Hematoxylin?
- A. Cell nuclei appear blue or purple ✅
- B. Cytoplasm appears pink
- C. Collagen fibers are highlighted
- D. Cells appear yellow
- Which stain is used to detect glycogen in tissue samples?
- A. Hematoxylin and eosin
- B. Periodic acid-Schiff (PAS) ✅
- C. Giemsa stain
- D. Masson’s trichrome
- What is the principle behind the use of immunohistochemistry (IHC) in histopathology?
- A. To visualize tissue structures with high magnification
- B. To detect specific antigens in tissue using labeled antibodies ✅
- C. To observe tissue under different lighting conditions
- D. To enhance tissue preservation
- Which technique is used to improve the contrast of tissue samples during microscopic examination?
- A. Fluorescence microscopy
- B. Phase contrast microscopy ✅
- C. Electron microscopy
- D. Bright field microscopy
- What is the role of enzyme histochemistry in histopathology?
- A. To visualize the enzymatic activity in tissue samples ✅
- B. To stain tissue sections
- C. To fix tissue for preservation
- D. To observe cells at a molecular level
