Histological Stains and Reagents MCQs
- What is the primary purpose of hematoxylin stain?
- A. To stain the cytoplasm
- B. To stain acidic structures (nucleus) ✅
- C. To stain muscle fibers
- D. To stain collagen
- What is eosin commonly used to stain?
- A. Nucleic acids
- B. Cytoplasm and extracellular matrix ✅
- C. Collagen fibers
- D. Lipids
- Which reagent is used for decalcification in tissue processing?
- A. Formalin
- B. Ethanol
- C. Hydrochloric acid ✅
- D. Xylene
- What is the function of Safranin in tissue staining?
- A. To stain cartilage and some bacteria ✅
- B. To stain the collagen fibers
- C. To enhance nuclear staining
- D. To differentiate between cell types
- Which stain is used to detect myelin in nervous tissue?
- A. Sudan black
- B. Luxol fast blue ✅
- C. Periodic acid-Schiff
- D. Wright’s stain
- Which reagent is used for clearing tissue in histopathology?
- A. Xylene ✅
- B. Acetone
- C. Alcohol
- D. Formalin
- The Gram stain is used to differentiate between:
- A. Gram-positive and Gram-negative bacteria ✅
- B. Red and white blood cells
- C. Nucleus and cytoplasm
- D. Epithelial and connective tissues
- What is the key feature of periodic acid-Schiff (PAS) stain?
- A. It stains mucopolysaccharides and glycoproteins ✅
- B. It highlights collagen fibers
- C. It stains acidic components of cells
- D. It is used to stain bacterial cell walls
- Which of the following is a special stain for detecting amyloid deposits?
- A. Congo red ✅
- B. Wright’s stain
- C. Gomori’s trichrome
- D. H&E stain
- Which chemical is used to prepare fixatives for histology?
- A. Formaldehyde ✅
- B. Ethanol
- C. Xylene
- D. Acetone
- What is the primary use of Wright’s stain in histology?
- A. To stain bacterial structures
- B. To stain blood smears and bone marrow specimens ✅
- C. To stain muscle fibers
- D. To stain the collagen fibers
- What does the Gomori trichrome stain highlight?
- A. Nuclei
- B. Collagen fibers ✅
- C. Muscle fibers
- D. Lipids
- The Masson’s trichrome stain is used to highlight:
- A. Muscle fibers and collagen ✅
- B. Lipids and fat cells
- C. Nucleus and cytoplasm
- D. Bacterial cell walls
- Which dye is used to stain acidic components of cells such as the nucleus?
- A. Eosin
- B. Hematoxylin ✅
- C. Safranin
- D. Methylene blue
- The Ziehl-Neelsen stain is primarily used to detect:
- A. Mycobacteria ✅
- B. Lipid deposits
- C. Fungal structures
- D. Collagen fibers
