Blood Banking MCQs:
These 300+ multiple-choice questions (MCQs) are designed to test your knowledge and understanding of various aspects of blood banking, a critical component of transfusion medicine. Blood banking involves the collection, processing, testing, storage, and distribution of blood and blood components to ensure their safe and effective use in medical treatments. The questions cover a wide range of topics, including blood typing, compatibility testing, transfusion reactions, blood component therapy, and the management of adverse events. By answering these questions, you will gain a deeper insight into the complexities and importance of blood banking in modern healthcare.
76. A family has been typed for HLA because 1 of the children needs a stem cell donor. Typing results are listed below:
What is the expected B antigen in child #3?
A. A1
B. A2
C. B12
D. B35 ✅
| Person | HLA Typing |
|---|---|
| father | A1,3;B8,35 |
| mother | A2,23;B12,18 |
| child#1 | A1,2;B8,12 |
| child#2 | A1,23;B8,18 |
| child#3 | A3,23;B18,? |
A. A1
B. A2
C. B12
D. B35 ✅
77. Which of the following is the best source of HLA-compatible platelets?
A. mother
B. father
C. siblings ✅
D. cousins
A. mother
B. father
C. siblings ✅
D. cousins
78. A patient is group O, Rh-negative with anti-D and anti-K in her serum. What percentage of the general Caucasian donor population would be compatible with this patient?
A. 0.5
B. 2.0
C. 3.0
D. 6.0 ✅
A. 0.5
B. 2.0
C. 3.0
D. 6.0 ✅
79. The observed phenotypes in a particular population are:
What is the gene frequency of Jka in this population?
A. 0.31
B. 0.45
C. 0.55
D. 0.60 ✅
| Phenotype | Number of persons |
|---|---|
| Jk(a+b-) | 122 |
| Jk(a+b+) | 194 |
| Jk(a-b+) | 84 |
A. 0.31
B. 0.45
C. 0.55
D. 0.60 ✅
80. In a random population, 16% of the people are Rh-negative (rr). What percentage of the Rh-positive population is heterozygous for r?
A. 36%
B. 48%
C. 57%
D. 66% ✅
A. 36%
B. 48%
C. 57%
D. 66% ✅
81. In relationship testing, a “direct exclusion” is established when a genetic marker is:
A. absent in the child, but present in the mother and alleged father
B. absent in the child, present in the mother and absent in the alleged father
C. present in the child, absent in the mother and present in the alleged father
D. present in the child, but absent in the mother and alleged father ✅
A. absent in the child, but present in the mother and alleged father
B. absent in the child, present in the mother and absent in the alleged father
C. present in the child, absent in the mother and present in the alleged father
D. present in the child, but absent in the mother and alleged father ✅
82. Relationship testing produces the following red cell phenotyping results:
What conclusions may be made?
A. there is no exclusion of paternity
B. paternity may be excluded on the basis of ABO typing
C. paternity may be excluded on the basis of Rh typing
D. paternity may be excluded on the basis of both ABO and Rh typing ✅
| ABO | Rh |
|---|---|
| alleged father: B | D+C-c+E+e |
| mother: O | D+C+E-c-e+ |
| child: O | D+C+E-c+e+ |
A. there is no exclusion of paternity
B. paternity may be excluded on the basis of ABO typing
C. paternity may be excluded on the basis of Rh typing
D. paternity may be excluded on the basis of both ABO and Rh typing ✅
83. In a relationship testing case, the child has a genetic marker that is absent in the mother and cannot be demonstrated in the alleged father. What type of paternity exclusion is this as?
A. indirect
B. direct ✅
C. prior probability
D. Hardy-weinberg
A. indirect
B. direct ✅
C. prior probability
D. Hardy-weinberg
84. A patient is typed with the following results:
The most probable reason for these findings is that the patient is group:
A. O; confusion due to faulty group O antiserum
B. O; with an anti-A1
C. Ax; with an anti-A1 ✅
D. A1; with an anti-A
| Patient’s cells with | Patient’s serum with | ||
|---|---|---|---|
| anti-A | 0 | A1 red cells | 2+ |
| anti-B | 0 | B red cells | 4+ |
| anti-A,B | 2+ | Ab screen | 0 |
A. O; confusion due to faulty group O antiserum
B. O; with an anti-A1
C. Ax; with an anti-A1 ✅
D. A1; with an anti-A
85. Human blood groups were discovered around 1900 by:
A. Jules Bordet
B. Louis Pasteur
C. Karl Landsteiner ✅
D. PL Mollison
A. Jules Bordet
B. Louis Pasteur
C. Karl Landsteiner ✅
D. PL Mollison
86. Cells of the A3 subgroup will:
A. react with Dolichos biflorus
B. bE-with anti-A
C. give a mixed-field reaction with anti-A,B ✅
D. bE-with anti-H
A. react with Dolichos biflorus
B. bE-with anti-A
C. give a mixed-field reaction with anti-A,B ✅
D. bE-with anti-H
87. The enzyme responsible for conferring H activity on the red cell membrane is alpha-:
A. galactosyl transferase
B. N-acetylgalactosaminyl transferase
C. L-fucosyl transferase ✅
D. N-acetylglucosaminyl transferase
A. galactosyl transferase
B. N-acetylgalactosaminyl transferase
C. L-fucosyl transferase ✅
D. N-acetylglucosaminyl transferase
88. Even in the absence of prior transfusion or pregnancy, individuals with the Bombay phenotype (Oh) will always have naturally occurring:
A. anti-Rh
B. anti-Ko
C. anti-U
D. anti-H ✅
A. anti-Rh
B. anti-Ko
C. anti-U
D. anti-H ✅
89. The antibody in the Lutheran system that is best detected at lower temperature is:
A. anti-Lua
B. anti-Lub
C. anti-Lu3
D. anti-Luab ✅
A. anti-Lua
B. anti-Lub
C. anti-Lu3
D. anti-Luab ✅
90. Which of the following antibodies is neutralizable by pooled human plasma?
A. anti-Kna
B. anti-Ch
C. anti-Yka
D. anti-Csa ✅
A. anti-Kna
B. anti-Ch
C. anti-Yka
D. anti-Csa ✅
91. Anti-Sda is strongly suspected if:
A. the patient has been previously transfused
B. the agglutinates are mixed-field and refractile
C. the patient is group A or B
D. only a small number of panel cells are reactive ✅
A. the patient has been previously transfused
B. the agglutinates are mixed-field and refractile
C. the patient is group A or B
D. only a small number of panel cells are reactive ✅
92. HLA antibodies are:
A. naturally occurring
B. induced by multiple transfusions ✅
C. directed against granulocyte antigens only
D. frequently cause hemolytic transfusion reactions
A. naturally occurring
B. induced by multiple transfusions ✅
C. directed against granulocyte antigens only
D. frequently cause hemolytic transfusion reactions
93. Genes of the major histocompatibility complex (MHC):
A. code for HLA-A, HLA-B, and HLA-C antigens only
B. are linked to genes in the ABO system
C. are the primary genetic sex-determinants
D. contribute to the coordination of cellular and humoral immunity ✅
A. code for HLA-A, HLA-B, and HLA-C antigens only
B. are linked to genes in the ABO system
C. are the primary genetic sex-determinants
D. contribute to the coordination of cellular and humoral immunity ✅
94. Isoimmunization to platelet antigen HPA-1a and the placental transfer of maternal antibodies would be expected to cause newborn:
A. erythroblastosis
B. leukocytosis
C. leucopenia
D. thrombocytopenia ✅
A. erythroblastosis
B. leukocytosis
C. leucopenia
D. thrombocytopenia ✅
95. Saliva from which of the following individuals would neutralize an auto anti-H in the serum of a group A, Le(a-b+) patient?
A. group A, Le (a-b-)
B. group A, Le (a+b-)
C. group O, Le (a+b-)
D. group O, Le (a-b+) ✅
A. group A, Le (a-b-)
B. group A, Le (a+b-)
C. group O, Le (a+b-)
D. group O, Le (a-b+) ✅
96. Inhibition testing can be used to confirm antibody specificity for which of the following antibodies?
A. anti-Lua
B. anti-M
C. anti-Lea
D. anti-Fya ✅
A. anti-Lua
B. anti-M
C. anti-Lea
D. anti-Fya ✅
97. Which of the following Rh antigens has the highest frequency in Caucasians?
A. D
B. E
C. c ✅
D. e
A. D
B. E
C. c ✅
D. e
98. Anti-D and anti-C are identified in the serum of a transfused pregnant woman, gravid 2, para 1. Nine months previously she received RH immune globulin (RhIg) after delivery. Tests of the patient, her husband, and the child revealed the following:
The most likely explanation for the presence of anti-C is that this antibody is:
A. actually anti-Cw
B. from the RhIg dose ✅
C. actually anti-G
D. naturally occurring
| anti-D | anti-C | anti-E | anti-c | anti-e | |
|---|---|---|---|---|---|
| patient | 0 | 0 | 0 | + | + |
| father | + | 0 | 0 | + | + |
| child | + | 0 | 0 | + | + |
A. actually anti-Cw
B. from the RhIg dose ✅
C. actually anti-G
D. naturally occurring
99. The phenomenon of an Rh positive person whose serum contains anti-D is best explained by:
A. gene deletion
B. missing antigen epitopes
C. trans position effect
D. gene inhibition ✅
A. gene deletion
B. missing antigen epitopes
C. trans position effect
D. gene inhibition ✅
100. When the red cells of an individual fail to react with anti-U, they usually fail to react with:
A. anti-M
B. anti-Leb
C. anti-S
D. anti-P1 ✅
A. anti-M
B. anti-Leb
C. anti-S
D. anti-P1 ✅
