Blood Banking MCQs:
These 300+ multiple-choice questions (MCQs) are designed to test your knowledge and understanding of various aspects of blood banking, a critical component of transfusion medicine. Blood banking involves the collection, processing, testing, storage, and distribution of blood and blood components to ensure their safe and effective use in medical treatments. The questions cover a wide range of topics, including blood typing, compatibility testing, transfusion reactions, blood component therapy, and the management of adverse events. By answering these questions, you will gain a deeper insight into the complexities and importance of blood banking in modern healthcare.

A. drug-dependent antibodies reacting with drug-treated cells
B. drug-dependent antibodies reacting in the presence of drug ✅
C. drug-independent with autoantibody production
D. nonimmunologic protein adsorption with positive DAT
A. causing rapid decay of complement components
B. chelating Mg++ ions, which prevents the assembly of C6
C. chelating Ca++ ions, which prevents assembly of C1 ✅
D. preventing chemotaxis
A. penicillin
B. cephalothin
C. methyldopa ✅
D. tetracycline
Test | Result |
---|---|
antibody screen | negative at AHG |
direct antiglobulin test | 3+ with anti-C3d |
eluate | negative |
A. warm autoimmune hemolytic anemia
B. cold agglutinin syndrome
C. paroxysmal cold hemoglobinuria
D. drug induced hemolytic anemia ✅
A. drug-dependent antibodies reacting with drug-treated cells
B. drug-dependent antibodies reacting in the presence of a drug ✅
C. drug-independent with autoantibody production
D. nonimmunologic protein adsorption with positive DAT
A. red cells were overwashed
B. centrifuge speed was set too high
C. residual patient serum inactivated the AHG reagent ✅
D. laboratorian did not add enough check cells
A. over-reading
B. IgG-coated screening cells
C. addition of an extra drop of serum
D. too heavy a cell suspension ✅
A. anti-A1
B. anti-A2
C. subgroups of A ✅
D. subgroups of B
A. deionized water
B. distilled water
C. normal saline
D. Alserver solution ✅
A. Fya ✅
B. E
C. S
D. M
Forward group: | Reverse Group: | ||||
---|---|---|---|---|---|
anti-A | anti-B | anti-D | Rh Control | A1 cells | B cells |
4+ | 2+ | 4+ | 0 | 0 | 3+ |
antibody screen: negative
ABO discrepancy was thought to be due to an antibody directed against a component of the typing sera. Which test would resolve this discrepancy?
A. A1 lectin
B. wash patient’s RBCs and repeat testing
C. anti-A,B and extend incubation of the reverse group
D. repeat reverse group using A2 cells ✅
A. reagent red cell panels
B. adsorption and elution ✅
C. titration and inhibition
D. cold autoadsorption
Test | Result |
---|---|
serum + P1 substance | negative |
serum + saline | negative |
A. anti-P1 is confirmed
B. anti-P1 is ruled out
C. a second antibody is suspected due to the results of the negative control
D. anti-P1 cannot be confirmed due to the results of the negative control ✅
A. inhibition ✅
B. dilution
C. complement fixation
D. hemolysis
Test | Result |
---|---|
tubes with patient serum + Lewis substance | 0 |
tubes with patient serum + saline control | + |
A. a second antibody is suspected due to the positive control
B. anti-Leb is confirmed because the tubes with Lewis substance are negative ✅
C. anti-Leb is not confirmed because the tubes with Lewis substance are negative
D. anti-Leb cannot be confirmed because the saline control is positive
Indicator cells | Sample | A | B | O |
---|---|---|---|---|
saliva plus anti-A | + | O | O | |
saliva plus anti-B | O | + | O | |
saliva plus anti-H | O | O | O |
A. group A secretor
B. group B secretor ✅
C. group AB secretor
D. group O secretor
Antibody specificity | Test results |
---|---|
anti-A | reactive |
anti-B | inhibited |
anti-H | inhibited |
A. A
B. AB
C. B ✅
D. O
A. a positive reaction ✅
B. a negative reaction
C. serum was not added
D. red cells have a positive direct antiglobulin test
A. transfusion of FFP affected the K typing ✅
B. wrong patient was drawn
C. results are normal
D. anti-K reagent was omitted on Monday
A. RBCs typing as weak D+
B. presence of red cell autoantibody
C. decreased serum bilirubin
D. agglutination with normal adult ABO compatible sera ✅
A. original reaction of rouleaux is confirmed
B. replacement test is invalid and should be repeated
C. original reaction was due to true agglutination ✅
D. antibody screen is negative
Test | Result |
---|---|
hemoglobin | 5g/dL |
reticulocyte count | 15% |
DAT | weak reactivity with poly-specific and anti-C3d, anti-IgG was negative |
antibody screen | negative |
Donath-Lansteiner test | positive; P cells showed no hemolysis |
A. paroxysmal cold hemoglobinuria (PCH) ✅
B. paroxysmal nocturnal hemoglobinuria (PNH)
C. warm autoimmune hemolytic anemia
D. hereditary erythroblastic multinuclearity with a positive acidified serum test (HEMPAS)
A. bromelin
B. chloroquine
C. LISS
D. DTT ✅
A. enzyme panel; select cell panel ✅
B. thiol reagents
C. lowering the pH and increasing the incubation time
D. using albumin as an enhancement media in combination with selective absorption