DHA Exam Questions for Lab Technician

microbiology

  1. Bacteria can cause pathogenesis to humans by:
    a) Capsular
    b) Secret enzymes
    c) Endogenous toxins
    d) Exogenous toxins
    e) All of the above ✅

  2. Function of pili of bacteria:
    a) Attachment to host tissue ✅
    b) Movement
    c) Reproduction (multiplication)
    d) Engulfment of food
    e) All of the above

  3. Bacteria motile gram-rods:
    a) Shigella
    b) Bordetella pertussis
    c) Pseudomonas aeruginosa ✅
    d) Yersinia pestis

  4. Strict aerobe bacteria:
    a) Mycobacterium ✅

  5. One is always non-motile gram-negative rods:
    a) Haemophilus influenzae
    b) Shigella ✅
    c) E. coli
    d) Salmonella
    e) Bordetella pertussis

  6. One is motile gram-negative rods:
    a) Haemophilus influenzae
    b) Bacillus anthracis
    c) Pseudomonas aerogenes ✅
    d) Vibrio cholerae
    e) Yersinia pestis

  7. One is gram-positive oval (cocci):
    a) Bacillus anthracis
    b) Meningococci ✅
    c) Pneumococci

  8. A child diagnosed with scarlet fever is caused by:
    a) Streptococcus pyogenes ✅
    b) Staphylococcus aureus

  9. Xylose lysine deoxycholate (XLD) is:
    a) Selective media
    b) Simple media
    c) Differential media ✅
    d) Enriched media
    e) Enrichment

  10. One is not a Romanowsky stain:
    a) Fields stain
    b) Gram stain ✅
    c) Giemsa stain
    d) Leishman stain

  11. Best sterilization of nutrient media is done by:
    a) Hot air oven
    b) Autoclaving ✅

  12. Organisms (bacteria) arranged in Chinese letters:
    a) Corynebacterium diphtheria ✅
    b) Bacillus anthracis

  13. Confirmatory test for Streptococcus pneumonia:
    a) Catalase
    b) Optochin disc ✅
    c) Coagulase
    d) Bile insolubility
    e) Bacitracin

  14. Gram-positive cocci arranged in groups (clusters):
    a) Streptococci
    b) Staphylococci ✅

  15. One is always oxidase-positive:
    a) Haemophilus influenzae
    b) Pseudomonas aeruginosa ✅

  16. Gram-negative bacteria color:
    a) Dark purple
    b) Pale to dark red ✅
    c) Orange

  17. Bacteria need a dark field to detect:
    a) T. pallidum (Syphilis) ✅

  18. Citrate test assesses:
    a) Mycobacteria
    b) Staph
    c) Strept
    d) Colistridia sp ✅

  19. Nutrient agar:
    a) Basic media ✅

  20. Disease caused by pyogenic streptococcus:
    a) Scarlet fever ✅

  21. Organism causing blood in stool:
    a) Shigella sp ✅

  22. Z.N stain used for diagnosis:
    a) Mycobacterium ✅

  23. Media used to differentiate between L.F and N.L.F:
    a) MacConkey media ✅

  24. Used for cleaning slides:
    a) Ethyl alcohol
    b) Methyl alcohol ✅

  25. The following is an ingredient of culture medium:
    a) Meat extract
    b) Mineral salts
    c) Agar
    d) Peptone
    e) None of the above
    f) All of the above ✅

  26. The most source of ATP in the cell is:
    a) Mitochondria ✅
    b) Cytoplasm
    c) Nucleus
    d) Cell wall

  27. H antigen is present in:
    a) Pili ✅
    b) Capsule
    c) Flagella
    d) Cell membrane

  28. Selective and differential medium of entero-pathogen is:
    a) Chocolate agar
    b) Blood agar
    c) DCA medium ✅
    d) Meat extract agar
    e) None of the above

  29. One of these is a prokaryotic cell:
    a) Fungi
    b) Bacteria ✅
    c) Entamoeba histolytica
    d) All of the above
    e) None of the above

  30. One of these organisms is gram-positive cocci arranged in pairs:
    a) Staphylococci
    b) Meningococci ✅
    c) Enterococci

  31. Substance used in catalyzing reactions:
    a) H2O2 ✅

  32. The best sample for the culture of children paralysis virus is:
    a) Anal swab ✅
    b) Blood culture
    c) Stool culture

  33. All of the following are true for Salmonella except:
    a) Motile
    b) Produces H2S
    c) Oxidase positive ✅

  34. The best media for urine culture is:
    a) Blood agar
    b) Chocolate agar
    c) CLED agar ✅

  35. The sterilization of autoclave is:
    a) 85°C for 30 minutes
    b) 150°C for 30 minutes
    c) 121°C for 15 minutes ✅

  36. Shigella sonnei colored in MacConkey & EMB:
    a) Colorless ✅
    b) Red
    c) Pink

  37. All the following bacteria are interobacter except:
    a) E. coli
    b) Proteus
    c) None of the previous ✅

  38. All the following parameters affect gram staining except:
    a) Use H2SO4
    b) Add absolute alcohol after washing
    c) Delaying the dryness of the slide ✅

  39. Blood sample is used to diagnose:
    a) C. tetani
    b) C. diphtheria
    c) None from them ✅

  40. Serious cause of food poisoning:
    a) Staph albus
    b) Salmonella typhi
    c) Salmonella enteritidis ✅

  41. Which of the following causes UTI & INDOL positive:
    a) Klebsiella
    b) Staphylococci
    c) E. coli ✅

  42. Which of the following culture media is suitable for semi-quantitative bacterial count in urine samples:
    a) McConkey agar
    b) Blood agar
    c) XLD medium
    d) Mannitol salt agar
    e) CLED medium ✅

  43. One of the following is capsulated bacteria:
    a) Streptococci
    b) E. coli
    c) Klebsiella pneumonia ✅

  44. The following organisms are lactose fermenters except:
    a) E. coli
    b) Enterobacter cloacae
    c) Shigella sonnei ✅
    d) Proteus spp.

  45. All is true about Enterobacteriaceae except:
    a) They are β-hemolytic and sorbitol
    b) Ferments Mannitol
    c) Grows in Methylene blue medium
    d) Inhibited growth with 6.5% NaCl and/or at temperature ✅

  46. All are true about Campylobacter jejuni except:
    a) Gram-negative curved bacilli
    b) Slow growth
    c) Grows on XLD medium ✅
    d) Arranged in pairs

  47. Beta hemolysis is enhanced when group B Streptococci is streaked at an angle in blood agar plate with:
    a) Streptococci
    b) Staph aureus ✅
    c) Micrococcus
    d) Streptococcus epidermidis
    e) Corynebacterium diphtheria

  48. All statements are true about Proteus mirabilis and Proteus vulgaris except:
    a) Exhibits swarming on BAP and McConkey’s agar
    b) Urease positive
    c) KCN and N2S positive
    d) Oxidase negative & liquefies gelatin ✅

  49. Klebsiella pneumonia:
    a) Capsulated ✅

  50. Some organisms are said to be pathogenic if they contain the following features:
    a) Coagulase
    b) Catalase
    c) Sugar
    d) Antibodies ✅

  51. Sterilization is best done by:
    a) 121°C, 15 PSI for 15-20 minutes ✅

  52. Organism soluble in bile:
    a) Staph
    b) Streptococci
    c) Pneumococci (Streptococcus pneumonia) ✅
    d) Haemophilus influenzae

  53. Thayer-Martin media is the choice for the isolation of the following organism:
    a) Pseudomonas aeruginosa
    b) Haemophilus influenzae
    c) Neisseria gonorrhoeae ✅

  54. Confirmatory test for Streptococcus pneumonia:
    a) Optochin sensitivity disc ✅
    b) Bacitracin disc sensitivity
    c) Bile solubility
    d) Catalase test

  55. Specimen suitable for microfilaria:
    a) Tissue
    b) Blood ✅
    c) Fluid

  56. Which of the following organisms is an anaerobic bacterium:
    a) Clostridium spp. ✅
    b) Haemophilus
    c) E. coli
    d) Yersinia enterocolitica

  57. Nutrient agar is:
    a) Selective media
    b) Differential media
    c) Special media
    d) Basic medium ✅

  58. The following organisms are encapsulated except:
    a) Pseudomonas aeruginosa ✅
    b) E. coli
    c) Haemophilus influenzae
    d) Streptococcus pneumonia

  59. Vi antigen is seen in:
    a) Capsule
    b) Pili
    c) Spore
    d) Flagella ✅

  60. All are true about Enterococci except:
    a) Have carbohydrate antigen of group D streptococci
    b) Positive aesculin hydrolysis
    c) Grow in the presence of bile salts
    d) Do not grow in the presence of 6.5% NaCl nor at 45°C ✅

  61. Significant bacteruria, pyuria, and alkaline urine probably indicate urinary tract infection due to:
    a) Staphylococcus epidermidis
    b) E. coli
    c) Proteus vulgaris ✅
    d) Pseudomonas aeruginosa
    e) Enterococcus faecalis

  62. Streptococci responsible for the majority of human infections are:
    a) Group A
    b) Beta-hemolytic streptococci
    c) Bacitracin-sensitive and CAMP (-)
    d) All of these ✅
    e) None of these

  63. To differentiate between Neisseria gonorrhoeae and Neisseria meningitidis:
    a) Fermentation of dextrose and lactose
    b) Fermentation of dextrose and maltose
    c) Fermentation of maltose and lactose ✅
    d) Fermentation of maltose and sucrose

  64. Blood culture is indicated in the following bacterial diseases except:
    a) Meningitis
    b) Endocarditis
    c) Gastroenteritis ✅
    d) Pyelonephritis
    e) Pneumonia

  65. Which of the following statements about Campylobacter jejuni is false:
    a) Gram-negative curved bacilli
    b) Arranged in pairs (see-gull)
    c) Slow growth
    d) Grows on XLD medium ✅
    e) Incubation temperature at 42°C

  66. The invasiveness of Streptococcus pneumoniae is due to the production of:
    a) Hemolysins
    b) Endotoxins
    c) Exotoxins
    d) Polysaccharide capsule ✅

  67. Agar is characterized by all the following except:
    a) Freezing point is 42°C & melting point is 100°C
    b) Nutritive
    c) Un-nutritive ✅

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