Gram-Negative Bacilli MCQs

  1. Which of the following is a characteristic of Gram-negative bacilli?
    • a) Thick peptidoglycan layer
    • b) Presence of an outer membrane containing lipopolysaccharides (LPS) ✅
    • c) Absence of a cell wall
    • d) Production of endospores

  2. Which Gram-negative bacillus is commonly associated with urinary tract infections (UTIs)?
    • a) Staphylococcus aureus
    • b) Escherichia coli ✅
    • c) Streptococcus pyogenes
    • d) Bacillus subtilis

  3. What distinguishes Pseudomonas aeruginosa from other Gram-negative bacilli?
    • a) Ability to ferment lactose
    • b) Production of blue-green pigments ✅
    • c) Resistance to oxygen
    • d) Lack of flagella

  4. Which Gram-negative bacillus is known for causing whooping cough?
    • a) Haemophilus influenzae
    • b) Legionella pneumophila
    • c) Bordetella pertussis ✅
    • d) Klebsiella pneumoniae

  5. Which Gram-negative bacillus is most often linked to gastroenteritis due to contaminated food and water?
    • a) Clostridium difficile
    • b) Helicobacter pylori
    • c) Salmonella enterica ✅
    • d) Bacillus anthracis

  6. What test is primarily used to differentiate lactose-fermenting Gram-negative bacilli from non-lactose fermenters?
    • a) Catalase test
    • b) MacConkey agar ✅
    • c) Coagulase test
    • d) Oxidase test

  7. Which of the following Gram-negative bacilli is a common cause of hospital-acquired infections, especially in immunocompromised patients?
    • a) Enterobacter cloacae ✅
    • b) Mycobacterium tuberculosis
    • c) Streptococcus pneumoniae
    • d) Bacillus cereus

  8. Which Gram-negative bacillus is the most common cause of traveler's diarrhea?
    • a) Escherichia coli ✅
    • b) Shigella dysenteriae
    • c) Vibrio cholerae
    • d) Yersinia pestis

  9. What is the primary mode of transmission for *Legionella pneumophila*, a Gram-negative bacillus that causes Legionnaires' disease?
    • a) Person-to-person contact
    • b) Ingestion of contaminated food
    • c) Inhalation of aerosolized water droplets ✅
    • d) Direct contact with infected soil

  10. Which of the following Gram-negative bacilli is associated with peptic ulcer disease and gastric cancer?
    • a) Campylobacter jejuni
    • b) Klebsiella oxytoca
    • c) Helicobacter pylori ✅
    • d) Proteus vulgaris

  11. What is a common characteristic of the Enterobacteriaceae family of Gram-negative bacilli?
    • a) Ability to form spores
    • b) Facultative anaerobes ✅
    • c) Strict aerobes
    • d) Lack of a cell wall

  12. Which Gram-negative bacillus is characterized by a "safety pin" appearance on microscopy?
    • a) Yersinia pestis ✅
    • b) Escherichia coli
    • c) Vibrio cholerae
    • d) Salmonella typhi

  13. What is the primary virulence factor of Vibrio cholerae, the causative agent of cholera?
    • a) Cholera toxin ✅
    • b) Endotoxin
    • c) Lecithinase
    • d) Capsule

  14. Which Gram-negative bacillus is frequently implicated in infections in patients with cystic fibrosis?
    • a) Haemophilus influenzae
    • b) Klebsiella pneumoniae
    • c) Pseudomonas aeruginosa ✅
    • d) Neisseria gonorrhoeae

  15. Which of the following is true about *Shigella* species?
    • a) They are motile with peritrichous flagella.
    • b) They are lactose fermenters.
    • c) They are primarily spread via the fecal-oral route. ✅
    • d) They are spore-forming bacteria.

  1. Which of the following Gram-negative bacilli is known for causing typhoid fever?
    • a) Escherichia coli
    • b) Salmonella typhi ✅
    • c) Pseudomonas aeruginosa
    • d) Haemophilus influenzae

  2. Which Gram-negative bacillus is responsible for causing plague?
    • a) Yersinia pestis ✅
    • b) Vibrio cholerae
    • c) Klebsiella pneumoniae
    • d) Neisseria meningitidis

  3. Which of the following is a common virulence factor of *Escherichia coli* that causes diarrhea?
    • a) Shiga toxin ✅
    • b) Cholera toxin
    • c) Botulinum toxin
    • d) Diphtheria toxin

  4. Which Gram-negative bacillus is associated with the development of meningitis in children?
    • a) Streptococcus pneumoniae
    • b) Haemophilus influenzae ✅
    • c) Staphylococcus aureus
    • d) Mycobacterium tuberculosis

  5. Which of the following Gram-negative bacilli is known for causing foodborne illness through the production of heat-stable enterotoxins?
    • a) Clostridium perfringens
    • b) Bacillus cereus
    • c) Staphylococcus aureus
    • d) Escherichia coli ✅

  6. Which Gram-negative bacillus is commonly found in the human gut and is a major cause of sepsis in neonates?
    • a) Klebsiella pneumoniae ✅
    • b) Pseudomonas aeruginosa
    • c) Salmonella enterica
    • d) Yersinia enterocolitica

  7. Which of the following Gram-negative bacilli is known for causing urinary tract infections (UTIs) and is often resistant to multiple antibiotics?
    • a) Proteus mirabilis
    • b) Enterococcus faecalis
    • c) Enterobacter cloacae ✅
    • d) Streptococcus agalactiae

  8. Which Gram-negative bacillus is responsible for causing the sexually transmitted infection (STI) gonorrhea?
    • a) Neisseria gonorrhoeae ✅
    • b) Chlamydia trachomatis
    • c) Treponema pallidum
    • d) Mycoplasma genitalium

  9. Which of the following Gram-negative bacilli is known for causing respiratory infections in individuals with chronic lung diseases?
    • a) Haemophilus influenzae
    • b) Streptococcus pneumoniae
    • c) Pseudomonas aeruginosa ✅
    • d) Mycobacterium tuberculosis

  10. Which Gram-negative bacillus is responsible for causing the zoonotic disease brucellosis?
    • a) Brucella melitensis ✅
    • b) Yersinia enterocolitica
    • c) Campylobacter jejuni
    • d) Vibrio vulnificus

  11. Which of the following Gram-negative bacilli is known for causing wound infections and is often associated with biofilm formation?
    • a) Staphylococcus aureus
    • b) Pseudomonas aeruginosa ✅
    • c) Streptococcus pyogenes
    • d) Clostridium tetani

  12. Which Gram-negative bacillus is responsible for causing the foodborne illness botulism?
    • a) Clostridium botulinum
    • b) Bacillus cereus
    • c) Staphylococcus aureus
    • d) Vibrio cholerae

  13. Which of the following Gram-negative bacilli is known for causing the sexually transmitted infection (STI) chancroid?
    • a) Haemophilus ducreyi ✅
    • b) Neisseria gonorrhoeae
    • c) Treponema pallidum
    • d) Chlamydia trachomatis

  14. Which Gram-negative bacillus is responsible for causing the zoonotic disease tularemia?
    • a) Francisella tularensis ✅
    • b) Yersinia pestis
    • c) Brucella abortus
    • d) Vibrio parahaemolyticus

  15. Which of the following Gram-negative bacilli is known for causing the foodborne illness listeriosis?
    • a) Listeria monocytogenes ✅
    • b) Staphylococcus aureus
    • c) Bacillus cereus
    • d) Clostridium perfringens
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