851. The cloned sheep “Dolly” had a genotype which is:
A. Haploid and identical to that of mother’s egg cell
B. Diploid & alike to that of mother’s somatic cells ✅
C. Diploid with the haploid set of chromosomes from the father and other from the mother
D. Diploid & alike to that of the donor’s somatic cells
A. Haploid and identical to that of mother’s egg cell
B. Diploid & alike to that of mother’s somatic cells ✅
C. Diploid with the haploid set of chromosomes from the father and other from the mother
D. Diploid & alike to that of the donor’s somatic cells
852. Pluripotent cells derived from the early pre-implantation of an embryo in mice are called:
A. Stem cells ✅
B. Organ culture
C. Somatic cell hybridization
D. Hybridoma
A. Stem cells ✅
B. Organ culture
C. Somatic cell hybridization
D. Hybridoma
853. A segment of DNA that reads from the same forward and backward is called:
A. Palindromic DNA ✅
B. Complementary DNA
C. Plasmid DNA
D. Copy DNA
A. Palindromic DNA ✅
B. Complementary DNA
C. Plasmid DNA
D. Copy DNA
854. The chemical nature of ‘humulin’ produced by recombinant DNA technology is:
A. Lipid
B. Protein ✅
C. Monosaccharide
D. Vitamin
A. Lipid
B. Protein ✅
C. Monosaccharide
D. Vitamin
855. Which of the following is associated with DNA fingerprinting?
A. Hybridoma
B. Site specific mutagenesis
C. Shotgun cloning
D. RFLP ✅
A. Hybridoma
B. Site specific mutagenesis
C. Shotgun cloning
D. RFLP ✅
856. Which technique would most likely be used to produce a large number of genetically identical offspring?
A. Cloning and in vitro culture ✅
B. Polymerase chain reaction
C. Chromatography
D. Electrophoresis
A. Cloning and in vitro culture ✅
B. Polymerase chain reaction
C. Chromatography
D. Electrophoresis
857. The restriction endonucleases are called so because:
A. They have a very restrictive or site specific endonuclease activity ✅
B. They cut DNA at a few restricted sites
C. They restrict the entry of foreign DNA into the cell by cleaving the DNA due to endonuclease activity
D. Their distribution is restricted to only some bacterial cells
A. They have a very restrictive or site specific endonuclease activity ✅
B. They cut DNA at a few restricted sites
C. They restrict the entry of foreign DNA into the cell by cleaving the DNA due to endonuclease activity
D. Their distribution is restricted to only some bacterial cells
858. A hybridoma cell:
A. Produces different types of antibodies against different types of antigens
B. Produces only specific antibodies only against a specific antigen ✅
C. Produces different types of antibodies but only one type of antigen
D. None of the above
A. Produces different types of antibodies against different types of antigens
B. Produces only specific antibodies only against a specific antigen ✅
C. Produces different types of antibodies but only one type of antigen
D. None of the above
859. A cancerous / myeloma cell in hybridoma helps in:
A. Continuous growth of hybridoma ✅
B. Production of antibodies
C. Both “A” and “B”
D. Neither “A” nor “B”
A. Continuous growth of hybridoma ✅
B. Production of antibodies
C. Both “A” and “B”
D. Neither “A” nor “B”
860. Which one of the following organism is used for the large scale production of recombinant insulin?
A. Plasmodium
B. Agrobacterium
C. Rhizobium
D. E. coli ✅
A. Plasmodium
B. Agrobacterium
C. Rhizobium
D. E. coli ✅
861. The unique feature of monoclonal antibody is that:
A. It is specific to a single antigenic determinant of a single antigen ✅
B. It is non-specific
C. It is specific to a few antigenic determinants
D. Restricted growth
A. It is specific to a single antigenic determinant of a single antigen ✅
B. It is non-specific
C. It is specific to a few antigenic determinants
D. Restricted growth
862. ‘Thermal Cycler’ is used in the reaction:
A. Enzyme linked immune-sorbant assay
B. Ligation reaction
C. Polymerase chain reaction ✅
D. Immobilization reaction
A. Enzyme linked immune-sorbant assay
B. Ligation reaction
C. Polymerase chain reaction ✅
D. Immobilization reaction
863. The technique of DNA finger printing is based on:
A. RFLP ✅
B. SNP
C. STR
D. All of the above
A. RFLP ✅
B. SNP
C. STR
D. All of the above
864. The term used for the technique used to separate DNA fragments based on their size is:
A. Centrifugation
B. Chromatography
C. Electrophoresis ✅
D. Hybridization
A. Centrifugation
B. Chromatography
C. Electrophoresis ✅
D. Hybridization
865. Which of the following is a method for introducing recombinant DNA into eukaryotic cells?
A. Microinjection ✅
B. Electroporation
C. Liposome-mediated transfer
D. All of the above
A. Microinjection ✅
B. Electroporation
C. Liposome-mediated transfer
D. All of the above
866. The process of using a virus to introduce new DNA into a cell is called:
A. Transformation
B. Transduction ✅
C. Conjugation
D. Infection
A. Transformation
B. Transduction ✅
C. Conjugation
D. Infection
867. Which of the following is a common application of genetic engineering?
A. Production of insulin ✅
B. Gene therapy
C. Creation of transgenic organisms
D. All of the above
A. Production of insulin ✅
B. Gene therapy
C. Creation of transgenic organisms
D. All of the above
868. In recombinant DNA technology, the term ‘vector’ refers to:
A. A virus
B. A plasmid ✅
C. A type of bacterium
D. A restriction enzyme
A. A virus
B. A plasmid ✅
C. A type of bacterium
D. A restriction enzyme
869. Gene therapy aims to:
A. Replace mutated genes ✅
B. Destroy cancer cells
C. Provide symptomatic relief
D. Cure infectious diseases
A. Replace mutated genes ✅
B. Destroy cancer cells
C. Provide symptomatic relief
D. Cure infectious diseases
870. The role of the ‘promoter’ in recombinant DNA is:
A. To initiate transcription ✅
B. To terminate transcription
C. To code for proteins
D. To facilitate translation
A. To initiate transcription ✅
B. To terminate transcription
C. To code for proteins
D. To facilitate translation
871. Which of the following statements about plasmids is TRUE?
A. Plasmids are found in all bacteria
B. Plasmids can replicate independently of chromosomal DNA ✅
C. Plasmids are essential for bacterial survival
D. Plasmids do not carry genes
A. Plasmids are found in all bacteria
B. Plasmids can replicate independently of chromosomal DNA ✅
C. Plasmids are essential for bacterial survival
D. Plasmids do not carry genes
872. The enzyme that cuts DNA at specific sites is called:
A. Ligase
B. Endonuclease ✅
C. Exonuclease
D. Polymerase
A. Ligase
B. Endonuclease ✅
C. Exonuclease
D. Polymerase
873. The main purpose of polymerase chain reaction (PCR) is:
A. To amplify DNA ✅
B. To sequence DNA
C. To cut DNA
D. To clone DNA
A. To amplify DNA ✅
B. To sequence DNA
C. To cut DNA
D. To clone DNA
874. Which of the following is NOT a method of genetic engineering?
A. Recombinant DNA technology
B. CRISPR-Cas9
C. Natural selection ✅
D. Gene cloning
A. Recombinant DNA technology
B. CRISPR-Cas9
C. Natural selection ✅
D. Gene cloning
875. The purpose of gel electrophoresis is to:
A. Amplify DNA
B. Separate DNA fragments ✅
C. Transform bacteria
D. Isolate plasmids
A. Amplify DNA
B. Separate DNA fragments ✅
C. Transform bacteria
D. Isolate plasmids
876. The presence of multiple copies of a gene in a genome is called:
A. Gene amplification ✅
B. Gene deletion
C. Gene conversion
D. Gene silencing
A. Gene amplification ✅
B. Gene deletion
C. Gene conversion
D. Gene silencing
877. Which of the following techniques is used to analyze gene expression?
A. Northern blotting ✅
B. Southern blotting
C. Western blotting
D. PCR
A. Northern blotting ✅
B. Southern blotting
C. Western blotting
D. PCR
878. The purpose of reverse transcription in genetic research is to:
A. Convert RNA to DNA ✅
B. Synthesize proteins
C. Amplify DNA
D. Separate DNA fragments
A. Convert RNA to DNA ✅
B. Synthesize proteins
C. Amplify DNA
D. Separate DNA fragments
879. Which of the following is used to identify specific DNA sequences?
A. Probe ✅
B. Primer
C. Ligase
D. Endonuclease
A. Probe ✅
B. Primer
C. Ligase
D. Endonuclease
880. The process of introducing a gene into an organism’s genome is called:
A. Transcription
B. Transformation ✅
C. Translation
D. Transduction
A. Transcription
B. Transformation ✅
C. Translation
D. Transduction
881. In DNA fingerprinting, what is the role of the restriction enzyme?
A. To amplify DNA
B. To cut DNA into fragments ✅
C. To sequence DNA
D. To ligate DNA
A. To amplify DNA
B. To cut DNA into fragments ✅
C. To sequence DNA
D. To ligate DNA
882. The type of cloning used to create Dolly the sheep is known as:
A. Gene cloning
B. Reproductive cloning ✅
C. Therapeutic cloning
D. Molecular cloning
A. Gene cloning
B. Reproductive cloning ✅
C. Therapeutic cloning
D. Molecular cloning
883. A plant or animal that has been genetically modified is called:
A. Transgenic ✅
B. Hybrid
C. Mutant
D. Cloned
A. Transgenic ✅
B. Hybrid
C. Mutant
D. Cloned
884. Which of the following is an application of genetic engineering in agriculture?
A. Herbicide resistance ✅
B. Pest resistance
C. Improved nutritional value
D. All of the above
A. Herbicide resistance ✅
B. Pest resistance
C. Improved nutritional value
D. All of the above
885. The main purpose of CRISPR technology is:
A. To sequence DNA
B. To cut DNA at specific locations ✅
C. To amplify DNA
D. To ligate DNA
A. To sequence DNA
B. To cut DNA at specific locations ✅
C. To amplify DNA
D. To ligate DNA
886. The process of creating a copy of a DNA molecule is known as:
A. Replication ✅
B. Transcription
C. Translation
D. Transformation
A. Replication ✅
B. Transcription
C. Translation
D. Transformation
887. In genetic engineering, a ‘probe’ is used for:
A. Cutting DNA
B. Amplifying DNA
C. Identifying specific DNA sequences ✅
D. Sequencing DNA
A. Cutting DNA
B. Amplifying DNA
C. Identifying specific DNA sequences ✅
D. Sequencing DNA
888. Which of the following is NOT a method for gene transfer in plants?
A. Agrobacterium-mediated transformation
B. Gene gun
C. Microinjection
D. Bacteriophage transduction ✅
A. Agrobacterium-mediated transformation
B. Gene gun
C. Microinjection
D. Bacteriophage transduction ✅
889. The main function of ligase in recombinant DNA technology is:
A. To cut DNA
B. To join DNA fragments ✅
C. To amplify DNA
D. To sequence DNA
A. To cut DNA
B. To join DNA fragments ✅
C. To amplify DNA
D. To sequence DNA
890. Which of the following statements about DNA is FALSE?
A. DNA is double-stranded
B. DNA contains uracil ✅
C. DNA is made up of nucleotides
D. DNA stores genetic information
A. DNA is double-stranded
B. DNA contains uracil ✅
C. DNA is made up of nucleotides
D. DNA stores genetic information
891. In genetic engineering, the term ‘expression vector’ refers to:
A. A vector used to insert a gene
B. A vector that expresses the inserted gene ✅
C. A vector that amplifies the gene
D. A vector that transfers the gene
A. A vector used to insert a gene
B. A vector that expresses the inserted gene ✅
C. A vector that amplifies the gene
D. A vector that transfers the gene
892. The use of transgenic animals is primarily aimed at:
A. Producing pharmaceuticals ✅
B. Creating pets
C. Enhancing growth
D. None of the above
A. Producing pharmaceuticals ✅
B. Creating pets
C. Enhancing growth
D. None of the above
893. The enzyme used to synthesize RNA from a DNA template is:
A. RNA polymerase ✅
B. DNA polymerase
C. Ligase
D. Reverse transcriptase
A. RNA polymerase ✅
B. DNA polymerase
C. Ligase
D. Reverse transcriptase
894. During DNA fingerprinting, the DNA obtained from samples can be multiplied into millions of copies by:
A. Autoradiography
B. Southern blotting
C. Polymerase chain reaction ✅
D. Electrophoresis
A. Autoradiography
B. Southern blotting
C. Polymerase chain reaction ✅
D. Electrophoresis
895. The first gene therapy was conducted in:
A. 1990 ✅
B. 1980
C. 2000
D. 1970
A. 1990 ✅
B. 1980
C. 2000
D. 1970
896. The process of introducing a gene into an organism’s genome is known as:
A. Transcription
B. Transformation ✅
C. Translation
D. Transduction
A. Transcription
B. Transformation ✅
C. Translation
D. Transduction
897. The term “genetic engineering” refers to:
A. The alteration of DNA ✅
B. The study of genes
C. The natural evolution of species
D. The selective breeding of animals
A. The alteration of DNA ✅
B. The study of genes
C. The natural evolution of species
D. The selective breeding of animals
898. The process of generating multiple copies of a DNA sequence is called:
A. Transcription
B. Amplification ✅
C. Translation
D. Transformation
A. Transcription
B. Amplification ✅
C. Translation
D. Transformation
899. The enzyme used to amplify DNA in PCR is:
A. DNA polymerase ✅
B. RNA polymerase
C. Ligase
D. Helicase
A. DNA polymerase ✅
B. RNA polymerase
C. Ligase
D. Helicase
900. Gene therapy, a method to cure inherited disease by:
A. Repairing the faulty gene
B. Introducing the correct copy of the gene ✅
C. Adding new cells to the body
D. Polymerase chain reaction
A. Repairing the faulty gene
B. Introducing the correct copy of the gene ✅
C. Adding new cells to the body
D. Polymerase chain reaction
